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Laboratory Research
Human amniotic membrane tissue was prepared using a proprietary process* including gentle cleansing, air-drying, and terminal sterilization. Biocompositional analyses were conducted using histological methods and multiplex protein arrays to quantify extracellular matrix (ECM) constituents, growth factors, angiogenic regulators, and cytokines/chemokines present in dHAM. To evaluate in vitro functional treatment effects, proliferation, IL-1β-induced inflammation, and TGF-β1-induced fibrosis assays were conducted using human dermal fibroblasts (HDFs).
Results:
The results demonstrate dHAM retains an intact ECM and a wide spectrum of signaling proteins that function to stimulate granulation, angiogenesis, and aid in reducing inflammation and fibrosis. Notably, dHAM treatment inhibits IL-1β-induced inflammatory protein expression, promotes HDF proliferation, and suppresses TGF-β1-induced gene expression.
Discussion:
AM’s therapeutic benefit in chronic wound care is associated with its ability to deliver soluble regulatory factors released from a biocompatible ECM reservoir to provide a favorable wound microenvironment. This study provides an in vitro characterization of air-dried dHAM, highlighting its multifaceted properties that aid in dermal wound healing mechanisms.