(LR-024) Wound Closure Adhesives on Full Thickness of Surgical Incisions

Sonja Gerdts, n/a; Shazia Siddiqui, n/a

Introduction: Suturing is a traditional wound closure method but needs a training to close the wounds efficiently. In addition, additional dressing method is required or triclosan coated suture is needed to prevent surgical site infections since wound lines still open to air after the suture closing.

Methods: Skin closure adhesives as 2-octyl cyanoacrylate monomer formulations afford similar wound closure strengths as 5.0 monofilament nylon suture while the cured polymer films work as a microbial barrier. In-vitro microbial strike through tests were performed against common five bacterial (3 for G(+) & 2 for G(-)) and two fungi strains on surgical site infections to investigate an efficacy of cured polymer film as the microbial barrier. Wound healing studies for 14-days implementation were also investigated on full thickness surgical incisions using both rat and swine models against market lead products without the suture closing.

Results: After 1-5 X 10⁶ CFU inoculations of each strain on the cured device and incubations at 37^oC for the bacterial plates or 25^oC for east/mold plates, it was confirmed that all trials (35 plates in total) showed no growth up to 14 days. On the swine model, all incisions (72 in total) were properly healed after the 14 days without wound dehiscence or infection symptom. The same clinical results were also demonstrated in the rat model after the 14 days implementation study.

Discussion: Seventy-two full thickness incisions were randomly closed using two different cyanoacrylate adhesives without suturing procedures. At three different end points after the surgery, overall animal health at 7 days, the device performances at14 days & local tissue responses at 14 days were respectively evaluated. Overall, both devices performed comparably to each other with regards to device safety and performance in the in-vivo rat and swine models respectively.
In-vitro study for microbial barriers, all strains on every plate didn't show any growth or color change of the media after 14 days incubation.